Virus:Article Title: DCAF14 promotes stalled fork stability to maintain genome integrity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-phospho-histone H2AX clone JBW301 Millipore Cat#05-636; RRID:AB_2755003 Rabbit polyclonal anti-53BP1 Abcam Cat#21083; RRID:AB_722496 Mouse monoclonal anti-PCNA Santa Cruz Biotechnology Cat#sc-56; RRID:AB_628110 Mouse monoclonal anti-CHK1 Santa Cruz Biotechnology Cat#sc-8408; RRID:AB_627257 Mouse monoclonal anti-SMARCAL1 Santa Cruz Biotechnology Cat#sc376377; RRID:AB_10987841 Rabbit polyclonal anti-ZRANB3 Bethyl Labs Cat#A303-033A; RRID:AB_10773114 Mouse monoclonal anti-BRCA2 Millipore Sigma Cat#OP95; RRID:AB_2067762 Mouse monoclonal anti-BRCA1 Santa Cruz Biotechnology Cat#sc6954; RRID:AB_626761 Mouse monoclonal anti-BLM Santa Cruz Biotechnology Cat#sc365753; RRID: AB_10851630 Rabbit monoclonal anti-KU70 Abcam Cat#ab92450; RRID:AB_10562280 Rabbit polyclonal anti-RAD51 Abcam Cat#ab63801; RRID:AB_1142428 Rabbit polyclonal anti-H3 Abcam Cat#ab1791; RRID:AB_302613 Mouse monoclonal anti-RPA32 Abcam Cat#ab2175; RRID:AB_302873 Rabbit polyclonal anti-phospho RPA32 (S4/S8) Bethyl Labs Cat#A300-245A; RRID:AB_210547 Rabbit polyclonal anti-PHIP Abcam Cat#ab86244; RRID:AB_1925318 Rabbit polyclonal anti-PHIP Novus Biologicals Cat#NBP2-33883 Rabbit polyclonal anti-RADX Novus Biologicals Cat#NBP2-13887; RRID:AB_2687552 Rabbit polyclonal anti-MRE11 Cell Signaling Technology Cat#4895S; RRID:AB_2145100 Rabbit polyclonal anti-DNA2 Invitrogen Cat#PA5-77943; RRID:AB_2735727 Rabbit polyclonal anti-CUL4A Abcam Catab72548; RRID:AB_1268363 Mouse monoclonal anti-CUL4B Bio-rad Cat#VMA00360 Rabbit polyclonal anti-DDB1 Cell Signaling Technology Cat#5428S; RRID:AB_10634753 Mouse anti-BrdU BD Biosciences Cat#347580; RRID:AB_10015219 Rat monoclonal anti-BrdU Abcam Cat#ab6326; RRID:AB_305426 Alexa Fluor 488 Azide Invitrogen Cat#A10266 Alexa Fluor 594 Azide Invitrogen Ca#A10270 Bacterial and virus strains DH5a Invitrogen Cat#18265017 Chemicals, peptides, and recombinant proteins ProLong Gold Antifade Mountant Invitrogen Cat#P36930 ProLong Gold Antifade Mountant with DAPI Invitrogen Cat#P36931 FuGENE HD Transfection Reagent Promega Cat#E2311 DharmaFECT 1 Transfection Reagent Dharmacon Cat#T-2001-03 Puromycin Dihydrochloride GIBCO Cat#A1113803 CldU Millipore Sigma Cat#C6891 IdU Millipore Sigma Cat#I7125 EdU Cayman Chemical Company Cat#20518 Propidium Iodide Invitrogen Cat#00-6990-50 Polyethylenimine Polysciences Inc Cat#23966 Hydroxyurea Millipore Sigma Cat#H8627 Camptothecin Selleckchem Cat#S1288 (Continued on next page) e1 Cell Reports 34, 108669, January 26, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Aphidicolin Millipore Sigma Cat#178273 Cisplatin USP Cat# 1134357 Olaparib Selleckchem Cat# S1060 Critical commercial assays Duolink In Situ Red Starter Kit Mouse/Rabbit Millipore Sigma Cat#DUO92101 Comet assay kit Trevigen Cat#4250-050-ESK Deposited Data Raw data This paper http://dx.doi.org/10.17632/2785zxsyzd.1 Experimental models: cell lines U2OS ATCC Cat#HTB-96; RRID:CVCL_0042 HEK293T ATCC Cat#CRL-3216; RRID:CVCL_0063 RPE-hTERT ATCC Cat#CRL-4000; RRID:CVCL_4388 DR-GFP U2OS Pierce et al., 1999 N/A Oligonucleotides DCAF14 siRNA Dharmacon Cat#J-019291-05 DCAF14 siRNA Dharmacon Cat#J-019291-06 DCAF14 siRNA Dharmacon Cat#J-019291-07 DCAF14 siRNA Dharmacon Cat#J-019291-08 DDB1 siRNA Dharmacon Cat#L-012890 RADX siRNA Dharmacon Cat#J-014634 RAD51 siRNA Dharmacon Cat#J-003530-11 RAD51 siRNA Dharmacon Cat#J-003530-12 MRE11 siRNA Dharmacon Cat#J-009271-08 SMARCAL1 siRNA Dharmacon Cat#J-013058-06 CUL4A siRNA Dharmacon Cat#L-012610 CUL4B siRNA Dharmacon Cat#L-017965 DNA2 siRNA Dharmacon Cat#D-026431-03 DNA2 siRNA Dharmacon Cat#D-026431-04 ZRANB3 siRNA Dharmacon Cat#D-010025-03 BRCA1 siRNA Dharmacon Cat#J-003461 BLM siRNA Dharmacon Cat#L-007287 BRCA2 siRNA QIAGEN Cat#SI02653434 All-stars negative control siRNA QIAGEN Cat#1027280 Recombinant DNA pSpCas9(BB)-2A-Puro (PX459) V2.0 Ran et al., 2013 Addgene Cat#62988 pCMV6-Entry Mammalian Expression Vector Origene Cat#PS100001 PHIP (NM_017934) Human Tagged ORF Clone Origene Cat#RC217114 CMV-hRad51 Rees et al., 2019 Addgene Cat#125570 CMV-hRad51(K133R) Rees et al., 2019 Addgene Cat#125571 Software and algorithms Graphpad Prism Graphpad software https://www.graphpad.com/scientific-software/ prism/ ImageJ NIH https://imagej.nih.gov/ij/download.html Cell Profiler Broad Institute https://cellprofiler.org/ Comet Score Tritek http://www.cometassay.com/index_files/ Page484.htm Cell Reports 34, 108669, January 26, 2021 e2
Recombinant:Article Title: DCAF14 promotes stalled fork stability to maintain genome integrity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-phospho-histone H2AX clone JBW301 Millipore Cat#05-636; RRID:AB_2755003 Rabbit polyclonal anti-53BP1 Abcam Cat#21083; RRID:AB_722496 Mouse monoclonal anti-PCNA Santa Cruz Biotechnology Cat#sc-56; RRID:AB_628110 Mouse monoclonal anti-CHK1 Santa Cruz Biotechnology Cat#sc-8408; RRID:AB_627257 Mouse monoclonal anti-SMARCAL1 Santa Cruz Biotechnology Cat#sc376377; RRID:AB_10987841 Rabbit polyclonal anti-ZRANB3 Bethyl Labs Cat#A303-033A; RRID:AB_10773114 Mouse monoclonal anti-BRCA2 Millipore Sigma Cat#OP95; RRID:AB_2067762 Mouse monoclonal anti-BRCA1 Santa Cruz Biotechnology Cat#sc6954; RRID:AB_626761 Mouse monoclonal anti-BLM Santa Cruz Biotechnology Cat#sc365753; RRID: AB_10851630 Rabbit monoclonal anti-KU70 Abcam Cat#ab92450; RRID:AB_10562280 Rabbit polyclonal anti-RAD51 Abcam Cat#ab63801; RRID:AB_1142428 Rabbit polyclonal anti-H3 Abcam Cat#ab1791; RRID:AB_302613 Mouse monoclonal anti-RPA32 Abcam Cat#ab2175; RRID:AB_302873 Rabbit polyclonal anti-phospho RPA32 (S4/S8) Bethyl Labs Cat#A300-245A; RRID:AB_210547 Rabbit polyclonal anti-PHIP Abcam Cat#ab86244; RRID:AB_1925318 Rabbit polyclonal anti-PHIP Novus Biologicals Cat#NBP2-33883 Rabbit polyclonal anti-RADX Novus Biologicals Cat#NBP2-13887; RRID:AB_2687552 Rabbit polyclonal anti-MRE11 Cell Signaling Technology Cat#4895S; RRID:AB_2145100 Rabbit polyclonal anti-DNA2 Invitrogen Cat#PA5-77943; RRID:AB_2735727 Rabbit polyclonal anti-CUL4A Abcam Catab72548; RRID:AB_1268363 Mouse monoclonal anti-CUL4B Bio-rad Cat#VMA00360 Rabbit polyclonal anti-DDB1 Cell Signaling Technology Cat#5428S; RRID:AB_10634753 Mouse anti-BrdU BD Biosciences Cat#347580; RRID:AB_10015219 Rat monoclonal anti-BrdU Abcam Cat#ab6326; RRID:AB_305426 Alexa Fluor 488 Azide Invitrogen Cat#A10266 Alexa Fluor 594 Azide Invitrogen Ca#A10270 Bacterial and virus strains DH5a Invitrogen Cat#18265017 Chemicals, peptides, and recombinant proteins ProLong Gold Antifade Mountant Invitrogen Cat#P36930 ProLong Gold Antifade Mountant with DAPI Invitrogen Cat#P36931 FuGENE HD Transfection Reagent Promega Cat#E2311 DharmaFECT 1 Transfection Reagent Dharmacon Cat#T-2001-03 Puromycin Dihydrochloride GIBCO Cat#A1113803 CldU Millipore Sigma Cat#C6891 IdU Millipore Sigma Cat#I7125 EdU Cayman Chemical Company Cat#20518 Propidium Iodide Invitrogen Cat#00-6990-50 Polyethylenimine Polysciences Inc Cat#23966 Hydroxyurea Millipore Sigma Cat#H8627 Camptothecin Selleckchem Cat#S1288 (Continued on next page) e1 Cell Reports 34, 108669, January 26, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Aphidicolin Millipore Sigma Cat#178273 Cisplatin USP Cat# 1134357 Olaparib Selleckchem Cat# S1060 Critical commercial assays Duolink In Situ Red Starter Kit Mouse/Rabbit Millipore Sigma Cat#DUO92101 Comet assay kit Trevigen Cat#4250-050-ESK Deposited Data Raw data This paper http://dx.doi.org/10.17632/2785zxsyzd.1 Experimental models: cell lines U2OS ATCC Cat#HTB-96; RRID:CVCL_0042 HEK293T ATCC Cat#CRL-3216; RRID:CVCL_0063 RPE-hTERT ATCC Cat#CRL-4000; RRID:CVCL_4388 DR-GFP U2OS Pierce et al., 1999 N/A Oligonucleotides DCAF14 siRNA Dharmacon Cat#J-019291-05 DCAF14 siRNA Dharmacon Cat#J-019291-06 DCAF14 siRNA Dharmacon Cat#J-019291-07 DCAF14 siRNA Dharmacon Cat#J-019291-08 DDB1 siRNA Dharmacon Cat#L-012890 RADX siRNA Dharmacon Cat#J-014634 RAD51 siRNA Dharmacon Cat#J-003530-11 RAD51 siRNA Dharmacon Cat#J-003530-12 MRE11 siRNA Dharmacon Cat#J-009271-08 SMARCAL1 siRNA Dharmacon Cat#J-013058-06 CUL4A siRNA Dharmacon Cat#L-012610 CUL4B siRNA Dharmacon Cat#L-017965 DNA2 siRNA Dharmacon Cat#D-026431-03 DNA2 siRNA Dharmacon Cat#D-026431-04 ZRANB3 siRNA Dharmacon Cat#D-010025-03 BRCA1 siRNA Dharmacon Cat#J-003461 BLM siRNA Dharmacon Cat#L-007287 BRCA2 siRNA QIAGEN Cat#SI02653434 All-stars negative control siRNA QIAGEN Cat#1027280 Recombinant DNA pSpCas9(BB)-2A-Puro (PX459) V2.0 Ran et al., 2013 Addgene Cat#62988 pCMV6-Entry Mammalian Expression Vector Origene Cat#PS100001 PHIP (NM_017934) Human Tagged ORF Clone Origene Cat#RC217114 CMV-hRad51 Rees et al., 2019 Addgene Cat#125570 CMV-hRad51(K133R) Rees et al., 2019 Addgene Cat#125571 Software and algorithms Graphpad Prism Graphpad software https://www.graphpad.com/scientific-software/ prism/ ImageJ NIH https://imagej.nih.gov/ij/download.html Cell Profiler Broad Institute https://cellprofiler.org/ Comet Score Tritek http://www.cometassay.com/index_files/ Page484.htm Cell Reports 34, 108669, January 26, 2021 e2
Transfection:Article Title: DCAF14 promotes stalled fork stability to maintain genome integrity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-phospho-histone H2AX clone JBW301 Millipore Cat#05-636; RRID:AB_2755003 Rabbit polyclonal anti-53BP1 Abcam Cat#21083; RRID:AB_722496 Mouse monoclonal anti-PCNA Santa Cruz Biotechnology Cat#sc-56; RRID:AB_628110 Mouse monoclonal anti-CHK1 Santa Cruz Biotechnology Cat#sc-8408; RRID:AB_627257 Mouse monoclonal anti-SMARCAL1 Santa Cruz Biotechnology Cat#sc376377; RRID:AB_10987841 Rabbit polyclonal anti-ZRANB3 Bethyl Labs Cat#A303-033A; RRID:AB_10773114 Mouse monoclonal anti-BRCA2 Millipore Sigma Cat#OP95; RRID:AB_2067762 Mouse monoclonal anti-BRCA1 Santa Cruz Biotechnology Cat#sc6954; RRID:AB_626761 Mouse monoclonal anti-BLM Santa Cruz Biotechnology Cat#sc365753; RRID: AB_10851630 Rabbit monoclonal anti-KU70 Abcam Cat#ab92450; RRID:AB_10562280 Rabbit polyclonal anti-RAD51 Abcam Cat#ab63801; RRID:AB_1142428 Rabbit polyclonal anti-H3 Abcam Cat#ab1791; RRID:AB_302613 Mouse monoclonal anti-RPA32 Abcam Cat#ab2175; RRID:AB_302873 Rabbit polyclonal anti-phospho RPA32 (S4/S8) Bethyl Labs Cat#A300-245A; RRID:AB_210547 Rabbit polyclonal anti-PHIP Abcam Cat#ab86244; RRID:AB_1925318 Rabbit polyclonal anti-PHIP Novus Biologicals Cat#NBP2-33883 Rabbit polyclonal anti-RADX Novus Biologicals Cat#NBP2-13887; RRID:AB_2687552 Rabbit polyclonal anti-MRE11 Cell Signaling Technology Cat#4895S; RRID:AB_2145100 Rabbit polyclonal anti-DNA2 Invitrogen Cat#PA5-77943; RRID:AB_2735727 Rabbit polyclonal anti-CUL4A Abcam Catab72548; RRID:AB_1268363 Mouse monoclonal anti-CUL4B Bio-rad Cat#VMA00360 Rabbit polyclonal anti-DDB1 Cell Signaling Technology Cat#5428S; RRID:AB_10634753 Mouse anti-BrdU BD Biosciences Cat#347580; RRID:AB_10015219 Rat monoclonal anti-BrdU Abcam Cat#ab6326; RRID:AB_305426 Alexa Fluor 488 Azide Invitrogen Cat#A10266 Alexa Fluor 594 Azide Invitrogen Ca#A10270 Bacterial and virus strains DH5a Invitrogen Cat#18265017 Chemicals, peptides, and recombinant proteins ProLong Gold Antifade Mountant Invitrogen Cat#P36930 ProLong Gold Antifade Mountant with DAPI Invitrogen Cat#P36931 FuGENE HD Transfection Reagent Promega Cat#E2311 DharmaFECT 1 Transfection Reagent Dharmacon Cat#T-2001-03 Puromycin Dihydrochloride GIBCO Cat#A1113803 CldU Millipore Sigma Cat#C6891 IdU Millipore Sigma Cat#I7125 EdU Cayman Chemical Company Cat#20518 Propidium Iodide Invitrogen Cat#00-6990-50 Polyethylenimine Polysciences Inc Cat#23966 Hydroxyurea Millipore Sigma Cat#H8627 Camptothecin Selleckchem Cat#S1288 (Continued on next page) e1 Cell Reports 34, 108669, January 26, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Aphidicolin Millipore Sigma Cat#178273 Cisplatin USP Cat# 1134357 Olaparib Selleckchem Cat# S1060 Critical commercial assays Duolink In Situ Red Starter Kit Mouse/Rabbit Millipore Sigma Cat#DUO92101 Comet assay kit Trevigen Cat#4250-050-ESK Deposited Data Raw data This paper http://dx.doi.org/10.17632/2785zxsyzd.1 Experimental models: cell lines U2OS ATCC Cat#HTB-96; RRID:CVCL_0042 HEK293T ATCC Cat#CRL-3216; RRID:CVCL_0063 RPE-hTERT ATCC Cat#CRL-4000; RRID:CVCL_4388 DR-GFP U2OS Pierce et al., 1999 N/A Oligonucleotides DCAF14 siRNA Dharmacon Cat#J-019291-05 DCAF14 siRNA Dharmacon Cat#J-019291-06 DCAF14 siRNA Dharmacon Cat#J-019291-07 DCAF14 siRNA Dharmacon Cat#J-019291-08 DDB1 siRNA Dharmacon Cat#L-012890 RADX siRNA Dharmacon Cat#J-014634 RAD51 siRNA Dharmacon Cat#J-003530-11 RAD51 siRNA Dharmacon Cat#J-003530-12 MRE11 siRNA Dharmacon Cat#J-009271-08 SMARCAL1 siRNA Dharmacon Cat#J-013058-06 CUL4A siRNA Dharmacon Cat#L-012610 CUL4B siRNA Dharmacon Cat#L-017965 DNA2 siRNA Dharmacon Cat#D-026431-03 DNA2 siRNA Dharmacon Cat#D-026431-04 ZRANB3 siRNA Dharmacon Cat#D-010025-03 BRCA1 siRNA Dharmacon Cat#J-003461 BLM siRNA Dharmacon Cat#L-007287 BRCA2 siRNA QIAGEN Cat#SI02653434 All-stars negative control siRNA QIAGEN Cat#1027280 Recombinant DNA pSpCas9(BB)-2A-Puro (PX459) V2.0 Ran et al., 2013 Addgene Cat#62988 pCMV6-Entry Mammalian Expression Vector Origene Cat#PS100001 PHIP (NM_017934) Human Tagged ORF Clone Origene Cat#RC217114 CMV-hRad51 Rees et al., 2019 Addgene Cat#125570 CMV-hRad51(K133R) Rees et al., 2019 Addgene Cat#125571 Software and algorithms Graphpad Prism Graphpad software https://www.graphpad.com/scientific-software/ prism/ ImageJ NIH https://imagej.nih.gov/ij/download.html Cell Profiler Broad Institute https://cellprofiler.org/ Comet Score Tritek http://www.cometassay.com/index_files/ Page484.htm Cell Reports 34, 108669, January 26, 2021 e2
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